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KMID : 0613820140240060694
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Journal of Life Science
2014 Volume.24 No. 6 p.694 ~ p.699
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Characterization Study of Crude Oil Degrading Microbiology Isolated from Incheon Bay
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Choi Hye-Jin
Oh Bo-Young
Han Young-Sun
Hur Myung-Je
Kim Jong-Guk
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Abstract
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Indigenous microorganisms play decisive roles in biodegradation. In this study, eighty strains of hydrocarbon-degrading microbes were isolated from Incheon Bay. Among them, 12 strains were selected by an oil film collapsing method. The bacterial strain ‘Incheon9’ was eventually selected based on its relatively higher lipase and emulsification activities, and was identified as Acinetobacter sp. (NCBI accession code: KF54854). The optimum condition for the growth and emulsification activity of Acinetobacter sp. Incheon9 was 20℃, pH 7, and 1% NaCl. The optimum time for the best production of biosurfactant was 72 hrs. The oil degradation ability of Acinetobacter sp. Incheon9 was investigated by measuring the residual oils in the culture medium by gas chromatography (FID). This research provides foundational data for eco-friendly environmental remediation by microorganisms.토착 미생물은 친환경적 정화에 중요한 역할을 한다.
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KEYWORD
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Acinetobacter sp., bioemulsifier, biosurfactant, lipase, oil-degradation
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